PageRuler™ pre-stained protein ladders are a mixture of ten blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
- Supplied in a loading buffer for direct loading on gels
- Colour-coded bands of similar intensity for easy visualisation
- Evaluated by SDS-PAGE and Western blotting
- Bright reference bands with orange at 70 and 25 kDa and green at 10 kDa
PageRuler™ pre-stained protein ladder is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents.
Dodatkowe informacje: Kit includes dye-stained proteins in 62,5 mM Tris-H3PO4 (pH 7,5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.