Drukuj

Chemiluminescencyjny zestaw RNA EMSA, LightShift™

Dostawca: Thermo Fisher Scientific
Pierce
PIER20158EA 4250 PLN
PIER20158
Chemiluminescencyjny zestaw RNA EMSA, LightShift™
Elektroforezy, Odczynniki Western Blotting Reagents
LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).

  • Chemiluminescent detection comparable to radioactive detection is sensitive
  • Develop X-ray films after 1 to 5 minute exposures thus being a time saving process
  • Compatible with RNA probes biotinylated by different methods
  • Eliminate waste concerns from radioactive RNA probes

Chemiluminescent RNA EMSA Kit is an in vitro technique for detection of protein-RNA interactions through changes in gel electrophoresis migration patterns. This kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods.

Dodatkowe informacje: Kit includes 125 nM biotinylated IRE RNA control (35 µl), 10 µM unlabeled IRE RNA control (25 µl), 2 mg/ml cytosolic liver extract (50 µl), 10 mg/ml tRNA (100 µl), 10X REMSA binding buffer (1 ml), 50% glycerol (500 µl), 2M KCl (500 µl), 1M MgCl2 (500 µl), DTT lyophilized, nuclease-free water (1,5 ml), 5X REMSA loading buffer (1 ml), stabilized streptavidin-HRP (1,5 ml), luminol/enhancer solution (80 ml), stable peroxide solution (80 ml), nucleic acid detection blocking buffer (500 ml), 4X wash buffer (500 ml), and substrate equilibration buffer (500 ml).
Zobacz ceny

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Więcej About VWR