Ascorbate oxidase (from Cucumis sp.)

Ascorbate Oxidase is an enzyme belonging to the family of oxidoreductases that catalyses the reaction: Ascorbic acid + ½ O₂ ascorbate oxidase → Dehydroascorbic acid + H₂O.

Enzyme Commission Number: E.C. 1.10.3.3
Isoelectric point (pI): between 6,0 and 7,8, 8,2 (Lit.)

Substrate specificity: The enzyme oxidises ascorbic acid and several ascorbic acid derivatives.

It is useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.

Unit definition: One unit will oxidise 1,0 µmole of L-ascorbate to dehydroascorbate per minute at pH 5,6 and 20 °C.

Stabilisers: Bovine serum albumin (BSA), borax, basic amino acids
Inhibitors: Azide, cyanide, Na₂S, diethyldithiocarbamate (Na)
Lyophilised powder desiccated at 2 to 8 °C

CAS No.:
9029-44-1
Source:
Cucumis sp.
Storage conditions:
2...8 °C
Form:
Light blue lyophilized powder
Enzyme name:
Ascorbate Oxidase
Enzyme activity:
1,000 - 3,000 unit/mg protein
Molecular weight:
~140 kDa
pH:
5,6