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Anti-POLK Rabbit Polyclonal Antibody

Dostawca: ProSci Inc.
POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

POLK antibody can be used for detection of POLK by ELISA at 1:62500. POLK antibody can be used for detection of POLK by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Antibody is purified by peptide affinity chromatography method.

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Antigen: POLK
Clonality: Polyclonal
Conjugation: Unconjugated
Host: Rabbit
Reactivity: Human, Mouse, Rat



Rozwiń / Zwiń
Rozmiar Ilość Numer katalogowy VWR Ilość
50 µg 50 µG PRSI26-347
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Antigen symbol POLK
Antigen name Polymerase (DNA Directed) Kappa
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Clonality Polyclonal
Host Rabbit
Western blot Yes
Antigen synonyms OTTHUMP00000020323, BETAN, RP11-529I10.1, DNA-directed DNA polymerase lambda, lambda, POLL, pol lambda, DNA polymerase kappa (DNA polymerase beta-N), pol beta2, polymerase (DNA-directed), DNA polymerase lamda2, polymerase (DNA directed), FLJ46002, DNA polymerase beta-2, BETA-N, POL-KAPPA, POLKAPPA
Immunogen A synthetic peptide corresponding a region of human POLK.
Form Lyophilized
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