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Anti-NSUN2 Rabbit Polyclonal Antibody

Dostawca: ProSci Inc.
Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).

NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Antibody is purified by peptide affinity chromatography method.

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type:
Antigen: NSUN2
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat




 

ZAMÓW

Rozwiń / Zwiń
Rozmiar Ilość Numer katalogowy VWR Ilość
50 µg 50 µG PRSI26-350
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SPECYFIKACJE

Antigen symbol NSUN2
Antigen name NOP2/Sun RNA methyltransferase family member 2p
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Clonality Polyclonal
Host Rabbit
Western blot Yes
ELISA Yes
Antigen synonyms proliferation-associated nucleolar protein p120, nucleolar protein 1 120kDa, NOL1, NOP120, putative ribosomal RNA methyltransferase NOP2, p120, NSUN1, nucleolar protein 2 homolog, NOL1/NOP2/Sun domain family member 1, proliferating-cell nucleolar antigen p120
Immunogen A synthetic peptide corresponding a region of human NSUN2.
Form Lyophilized
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