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Anti-NEK3 Rabbit Polyclonal Antibody

Dostawca: ProSci Inc.
NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.

NEK3 antibody can be used for detection of NEK3 by ELISA at 1:1562500. NEK3 antibody can be used for detection of NEK3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Antibody is purified by peptide affinity chromatography method.

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type:
Antigen: NEK3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human




 

ZAMÓW

Rozwiń / Zwiń
Rozmiar Ilość Numer katalogowy VWR Ilość
50 µg 50 µG PRSI26-375
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SPECYFIKACJE

Antigen symbol NEK3
Antigen name NIMA-related kinase 3
Reactivity Human
Conjugation Unconjugated
Clonality Polyclonal
Host Rabbit
Western blot Yes
ELISA Yes
Antigen synonyms HSPK36
Immunogen A synthetic peptide corresponding a region of human NEK3.
Form Lyophilized
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