- Pk:50 µG
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Storage conditions:Lyophilized protein should be stored at −20 °C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 °C for 2-7 days. Aliquots of reconstituted samples are stable at −20 °C for 3 months.
- Biological activity:ED50 is less than 0.1 ng/ml as determined by the dose-dependent stimulation of human TF-1 cell proliferation.
Specific Activity of 1.0 x 107 IU/ mg. - Protein synonyms:Molgramostin|GMCSF|Sargramostim|Granulocyte-Macrophage Colony-Stimulating Factor|GM-CSF|Colony-Stimulating Factor|CSF2|CSF
- Protein/peptide name:GMCSF
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:14.6 kD
- Sequence:Ala18-Glu144
- Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the Lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.
- This recombinant protein can be used for biological assays. For research use only.