Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Cynomolgus monkey
• Tag sequence:
  His Tag (SPR & BLI & HPLC verified)
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method
• Biological activity:
  Captured Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. ACRBCDA-C52H7) on CM5 Chip via Anti-His antibody can bind Rituximab with an affinity constant of 4,18 μM as determined in SPR assay (Biacore T200) (QC tested). Loaded Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. ACRBCDA-C52H7) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 3,3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Rituximab on FAB2G Biosensor, can bind Cynomolgus CD32a, His Tag (SPR & BLI & HPLC verified) (Cat. No. ACRBCDA-C52H7) with an affinity constant of 6,0 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)
• Gene ID:
  Q8SPW4-1
• Protein synonyms:
  CD32|CD32a|IGFR2|FCGR2A1|FCG2 |FCGR2A
• Protein/peptide name:
  Fc gamma RIIA/ CD32a
• Purity:
  >90% as determined by reduced SDS-PAGE.
• Molecular weight:
  22,1 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation
• Shipping temperature:
  Powder, RT