Supelco® Discovery® BIO RP18e Reversed-Phase Solution to Protein and Peptide Separation Challenges

Supplier: Merck

Supplier: Merck

About this item

Discovery® BIO Wide Pore reversed-phase columns satisfy the need for efficiency, selectivity, scalability, and reproducibility for RP HPLC analyses of proteins, peptides, and small biomolecules.

Better protein and peptide resolution compared to leading RP-HPLC FPP columns
High efficiency for peptide mapping
Low-bleed, inert surface chemistry make them ideal for LC-MS applications
Reliable reproducibility run -to-run, column-to-column, batch-to-batch
Separation are completely scalable from analytical to preparative dimensions
Surface coverage: 3,6 µmol/m²

Discovery® BIO Wide Pore HPLC columns are packed with C18 ligands bonded to 3, 5, or 10 µm spherical, high purity silica particles containing 300 Å pores.

The low-bleed character and excellent peak shape without TFA in the mobile phase makes these columns ideal for proteomics and other LC-MS applications and preparative purification.

Delivery: Column test certificate available inside the box.

Specifications

Catalog No:
567271-U
567270-U
568272-U
568271-U
567208-U
567207-U
568230-U
568223-U
567200-U
568213-U
568203-U
567209-U
567205-U
568273-U
567204-U
568222-U
567212-U
567202-U
567211-U
567201-U

Application:
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules
Peptide mapping/proteolytic digests/small biomolecules

Carbon load:
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%
9,2%

Endcapped:
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes

Max. pressure:
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar
400 bar

pH range:
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8
2 - 8

Surface area:
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g
100 m²/g

Temperature limit:
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C
0...70 °C

USP code:
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1
L1

Shipping conditions:
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water
Acetonitril/water

Specifications

Analytical columns, particle size 3 µm

Product Information

Catalog #
Supplier #
Column Format
Phase
Pore size
Particle Size
Ø int.
Availability
Price
HPLC column
HPLC column
567200-U
567200-U
Analytical
C18e
300 Å
3 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 3 130 zł
3 130 zł
1 * 1 Szt.
3 130 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567205-U
567205-U
Analytical
C18e
300 Å
3 µm
4,6 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 920 zł
4 920 zł
1 * 1 Szt.
4 920 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567204-U
567204-U
Analytical
C18e
300 Å
3 µm
4,6 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 480 zł
4 480 zł
1 * 1 Szt.
4 480 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567202-U
567202-U
Analytical
C18e
300 Å
3 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 920 zł
4 920 zł
1 * 1 Szt.
4 920 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567201-U
567201-U
Analytical
C18e
300 Å
3 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 480 zł
4 480 zł
1 * 1 Szt.
4 480 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water

Guard cartridges, particle size 3 µm

Product Information

Catalog #
Supplier #
Column Format
Phase
Pore size
Particle Size
Ø int.
Availability
Price
HPLC guard cartridge (pack of 2 each)
HPLC guard cartridge (pack of 2 each)
567270-U
567270-U
Guard cartridge
C18e
300 Å
3 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 1 270 zł
1 270 zł
1 * 1 Szt.
1 270 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC guard cartridge kit
HPLC guard cartridge kit
567271-U
567271-U
Guard cartridge
C18e
300 Å
3 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 1 140 zł
1 140 zł
1 * 1 Szt.
1 140 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water

Analytical columns, particle size 5 µm

Product Information

Catalog #
Supplier #
Column Format
Phase
Pore size
Particle Size
Ø int.
Availability
Price
HPLC column
HPLC column
568203-U
568203-U
Analytical
C18e
300 Å
5 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 480 zł
4 480 zł
1 * 1 Szt.
4 480 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
568213-U
568213-U
Analytical
C18e
300 Å
5 µm
4,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 480 zł
4 480 zł
1 * 1 Szt.
4 480 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
568223-U
568223-U
Analytical
C18e
300 Å
5 µm
4,6 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 480 zł
4 480 zł
1 * 1 Szt.
4 480 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
568230-U
568230-U
Analytical
C18e
300 Å
5 µm
10,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 9 860 zł
9 860 zł
1 * 1 Szt.
9 860 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
568222-U
568222-U
Analytical
C18e
300 Å
5 µm
4,6 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 4 110 zł
4 110 zł
1 * 1 Szt.
4 110 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water

Guard cartridges, particle size 5 µm

Product Information

Catalog #
Supplier #
Column Format
Phase
Pore size
Particle Size
Ø int.
Availability
Price
HPLC guard cartridge (1 pack of 2 each; 2 guard columns)
HPLC guard cartridge (1 pack of 2 each; 2 guard columns)
568272-U
568272-U
Guard cartridge
C18e
300 Å
5 µm
4,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 1 270 zł
1 270 zł
1 * 1 Szt.
1 270 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC guard cartridge (kit contains 1 guard column and a stand-alonge holder, a piece of tubing and 2 nuts and ferrules)
HPLC guard cartridge (kit contains 1 guard column and a stand-alonge holder, a piece of tubing and 2 nuts and ferrules)
568271-U
568271-U
Guard cartridge
C18e
300 Å
5 µm
2,1 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 1 140 zł
1 140 zł
1 * 1 Szt.
1 140 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC guard cartridge (kit contains 1 guard column and a stand-alonge holder, a piece of tubing and 2 nuts and ferrules)
HPLC guard cartridge (kit contains 1 guard column and a stand-alonge holder, a piece of tubing and 2 nuts and ferrules)
568273-U
568273-U
Guard cartridge
C18e
300 Å
5 µm
4,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 1 270 zł
1 270 zł
1 * 1 Szt.
1 270 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water

Analytical columns, particle size 10 µm

Product Information

Catalog #
Supplier #
Column Format
Phase
Pore size
Particle Size
Ø int.
Availability
Price
HPLC column
HPLC column
567207-U
567207-U
Analytical
C18e
300 Å
10 µm
10,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt.
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567208-U
567208-U
Analytical
C18e
300 Å
10 µm
10,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 7 490 zł
7 490 zł
1 * 1 Szt.
7 490 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567209-U
567209-U
Analytical
C18e
300 Å
10 µm
10,0 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 9 170 zł
9 170 zł
1 * 1 Szt.
9 170 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water

Preparative columns, particle size 10 µm

Product Information

Catalog #
Supplier #
Column Format
Phase
Pore size
Particle Size
Ø int.
Availability
Price
HPLC column
HPLC column
567212-U
567212-U
Preparative
C18e
300 Å
10 µm
21,2 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt. - 17 020 zł
17 020 zł
1 * 1 Szt.
17 020 zł
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water
HPLC column
HPLC column
567211-U
567211-U
Preparative
C18e
300 Å
10 µm
21,2 mm
Log in for availability
Log in for availability
1 * 1 Szt.
1 * 1 Szt.
Specifications:
More
Specifications
Environmentally Preferable
Application
Peptide mapping/proteolytic digests/small biomolecules
Carbon load
9,2%
Endcapped
Yes
Max. pressure
400 bar
pH range
2 - 8
Surface area
100 m²/g
Temperature limit
0...70 °C
USP code
L1
Shipping conditions
Acetonitril/water

About this item

Specifications

Specifications

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