The Vivapure® AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for in vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.
- Fast and easy virus purification: Purification typically completed in 2 hours; >10× faster alternative to CsCl density gradient
- Quantitative yields: In contrast to CsCl density gradient, the complete cell culture is used for virus purification and not only the viral pellet
- Flexible product range: Applicable from initial construct screening to large scale virus production
- Complete kit: Including filtration devices, AdenoPACK units for virus purification, Vivaspin and all buffers
- Low endotoxin levels: High cell viability and infection rates due to endotoxin levels of <0,025 EU/ml
Vivapure® AdenoPACK™ 20/20 RT* is the downscale kit in the AdenoPACK series, purifying up to 1×10¹² viral particles from 20 ml cell culture. Especially
when testing new constructs, parallel and fast purifications of different adenoviruses are essential. This kit allows the rapid, simple and affordable
spin column based purification of 6 different samples in parallel and bridges a gap in the CsCl density gradient method.
Vivapure® AdenoPACK™ 100/100 RT is optimally suited for adenovirus purification for up to 200 ml cell culture for in vitro transfection. This flexible kit contains two AdenoPACK 100 units, which can be either used in tandem for the purification of up to 200 ml cell culture for recovering 1×10¹³ viral particles or individually for purifying 1 to 3×10¹² viral particles from up to 60 ml cell culture. The purification is pursued manually with a syringe optimally
attached to a retort stand. However, for even more convenience, protocols are provided for optionally running the virus purification with a peristaltic pump or with an infusion pump, in additional to detailed instructions for a manual operation supplied with the kit. The accessories needed for the operation with a pump are supplied as individual products.
Vivapure® AdenoPACK™ 500/500 RT is the direct upscale kit to the AdenoPACK 100, for adenovirus purification. In only 2 hours up to 3×10¹³ adenovirus particles are purified and concentrated from 500 ml cell culture. This completely ready to use kit is conveniently operated by a laboratory pump, offering optimal flow control and minimal hands-on time. This easy to use product replaces lengthy and inefficient cesium chloride density gradient methods.
*RT kit does not contain Benzonase® DNA-digesting enzyme.
