Corning® Nu-Serum™ IV is a growth medium supplement that provides a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture.
The low-protein content facilitates protein purification, virus production (Okada, M., et al., Microbiological Immunol.).
Tested at a concentration of 10% for the ability to stimulate a ≧100-fold increase in growth of BHK-21 and a ≧13-fold increase in growth of BALB/c-3T3 cells. Tested for total protein content, pH, and osmolarity (information included with product). Filtered (0.2 µm membrane) and tested and found negative for the presence of bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption. Tested for endotoxin (LAL assay).
31:657, 1987), monoclonal antibody production and screening (Sjogren-Janssen, E., e al., J. Immunol. Meth. 84:357, 1985), and increases the frequency of successful transfection of cells (Seed, B., et al., Proc. Natl. Acad. Sci. USA 84:3365, 1987; Duval, N., et al., J. Cell Biol. 118:641, 1992.
- Environmentally Preferable:Low Manufacturing Impact
- Environmental certification:ISO 14001 - Bedford
- Formulation:Frozen solution containing 2.5% foetal bovine serum, EGF, ECGS, insulin, human transferring, triiodothyronine, progesterone, oestradiol-17ß, testosterone, hydrocortisone, selenous acid, o-phosphorylethanolamine, glucose, amino acids, vitamins, and other trace elements and nutrients contained in the Ham's F12 medium base
- Quality control:Tested at a concentration of 10% for the ability to stimulate a 100-fold increase in growth of BHK-21 and a 13-fold increase in growth of BALB/c-3T3 cells
Tested for total protein content, pH, and osmolarity
Filtered (0,2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and haemadsorption
Tested for endotoxin (LAL assay) - Recommended concentration:Replaces foetal bovine and other sera on an equivalent volume basis
- Storage and stability:Stable for at least three months at −20 °C
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- Corning® Nu-Serum™ IV
Low Manufacturing Impact - Corning® Nu-Serum™ IVCorning® Nu-Serum™ IV355504500 mL1 * 500 mL2 200 zł
Low Manufacturing Impact