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RevertAid™Zestaw do syntez H Minus Reverse Transcriptase

Dostawca: Fermentas (Thermo Scientific)
Fermentas
EP0451EA 839 PLN
EP0451 EP0452
RevertAid™Zestaw do syntez H Minus Reverse Transcriptase
Odczynniki do kwasów nukleinowych Odczynniki do RT-PCR, syntezy cDNA
RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

  • High yields of full length first strand cDNA up to 13 kb
  • Optimum activity at 42 to 45 °C
  • Active up to 55 °C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labelled nucleotides)

Informacja o dostawie: Supplied with 5X reaction buffer.
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