Autoinduction systems, Overnight Express™ Autoinduction System 1
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ Autoinduction System 2
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ NMR Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ Instant LB and TB Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Pierce™ Cell-free Protein Expression Kits, 1-Step Human High Yield Maxi IVT Kit
Supplier: Thermo Fisher Scientific
The 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per ml than other mammalian IVT systems.
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Cell-free protein expression kits, 1-step human high yield mini IVT kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.
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Cell-free protein expression kits, 1-step human coupled IVT kit - DNA
Supplier: Thermo Fisher Scientific
The 1-Step Human Coupled IVT Kit – DNA is a mammalian in vitro translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.
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Rapid translation system (RTS), wheat germ kits
Supplier: biotechrabbit
The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.
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Rapid translation system (RTS), E. coli high yield kits
Supplier: biotechrabbit
The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.
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Rapid translation system (RTS), E. coli disulfide and fab kits
Supplier: biotechrabbit
The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.
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Cell-free protein synthesis, rapid translation system (RTS) linear template kits
Supplier: biotechrabbit
Expression kits for PCR-based generation of linear templates.
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Rapid translation system (RTS), insect membrane kits
Supplier: biotechrabbit
RTS 100 Insect Membrane Kit provides high yields of in vitro synthesised post-translationally modified eukaryotic proteins.
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Silver Protein strong, powder for histology
Supplier: Thermo Fisher Scientific
Silver protein is used in electron microscopy with periodic acid and thiocarbohydrazide or thiosemicarbohydrazide as a positive stain for carbohydrates such as glycogen. It can also be used for light microscopy to stain nerve tissue. It is also used in histological stain for microscopic analysis.
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TriLink® CleanCap® M6 Cas9 mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.
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TriLink® CleanCap® Cas9 mRNA
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.
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TriLink® CleanCap® Cas9 mRNA (5 moU)
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.
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TriLink® CleanCap® M6 FLuc mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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TriLink® CleanCap® FLuc mRNA (5 moU)
Supplier: TriLink BioTechnologies
The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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TriLink® CleanCap® Cas9 Nickase mRNA (5 moU)
Supplier: TriLink BioTechnologies
Cas9 Nickase mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.
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Rapid mouse antibody isotyping kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Rapid isotyping kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.
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TriLink® CleanCap® mCherry mRNA (5 moU)
Supplier: TriLink BioTechnologies
mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.
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CleanCap® AU EGFP saRNA
Supplier: TriLink BioTechnologies
This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish Aequorea victoria, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.
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CleanCap® AU FLuc saRNA
Supplier: TriLink BioTechnologies
This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly Photinus pyralis, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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TriLink® CleanCap® EGFP mRNA
Supplier: TriLink BioTechnologies
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
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TriLink® CleanCap® Beta gal mRNA (5moU)
Supplier: TriLink BioTechnologies
β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.
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TriLink® CleanCap® EPO mRNA (5moU)
Supplier: TriLink BioTechnologies
EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.
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TriLink® CleanCap® M6 mCherry mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.
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ProLite™ His-Tag Protein Gel Staining Kit
Supplier: AAT Bioquest
This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.
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TriLink® CleanCap® FLuc mRNA
Supplier: TriLink BioTechnologies
The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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TriLink® CleanCap® M6 EGFP mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.